Molecular engineering of G-quadruplex ligands based on solvent effect of polyethylene glycol
نویسندگان
چکیده
Because various non-parallel G-quadruplexes of human telomeric sequences in K+ solution can be converted to a parallel G-quadruplex by adding polyethylene glycol (PEG) as a co-solvent, we have taken advantage of this property of PEG to study the covalent attachment of a PEG unit to a G-quadruplex ligand, 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC). The hybrid ligand with the PEG unit, BMVC-8C3O or BMVC-6C2O by substituting either the tetraethylene glycol or the triethylene glycol terminated with a methyl-piperidinium cation in N-9 position of BMVC, not only induces structural change from different non-parallel G-quadruplexes to a parallel G-quadruplex but also increases the melting temperature of human telomeres in K+ solution by more than 45°C. In addition, our ligand work provides further confidence that the local water structure plays the key to induce conformational change of human telomere.
منابع مشابه
The Effect of Polyethylene Glycol and Nanoclay on the Rheological Behavior of Dispersing Cationic Polyurethane Nanocomposites
Cationic waterborne polyurethane as a new dispersing polymer was synthesized by using relatively hydrophilic polyols. Dispersing cationic polyurethane (DCPU) nanocomposites were prepared using isophorone diisocyanate (IPDI), polyethylene glycol (PEG) with different molecular weights (Mn=200, 400, 600,and1000 g/mol), N-methyl diethanolamine (MDEA), dibutyltin dilaurate (DBTDL) and different perc...
متن کاملSurface Adsorption of Polyethylene Glycol and Polyvinyl Alcohol with Variable Molecular Weights on Zinc Oxide Nanoparticles
متن کامل
Complicated behavior of G-quadruplexes and evaluating G-quadruplexes' ligands in various systems mimicking cellular circumstance
Environments surrounding G-rich sequences remarkably affect the conformations of these structures. A proper evaluation system mimicking the crowded environment in a cell with macromolecules should be developed to perform structural and functional studies on G-quadruplexes. In this study, the topology and stability of a G-quadruplex formed by human telomeric repeat sequences were investigated in...
متن کاملEffect of Polyethylene Glycol and Triton X-100 on the Enzymatic Treatment of Bisphenol A
The current study includes the screening of indigenous extracellular laccase producing fungus from the soil, identified as Phanerochaete chrysosporiumspecies.The selected strain was applied under the optimized condition for the production of laccase in the solid state fermentation. The effect of polyethylene glycol and Triton X-100 on the enhancement of enzyme activity and bi...
متن کاملEco-friendly Self-curing Concrete Incorporated with Polyethylene Glycol as Self-curing Agent (RESEARCH NOTE)
Today concrete is most generally utilized development material in the world due to its strength and sturdiness properties. To attain good strength, curing of concrete is important so we introduce the concept of self-curing concrete rather than immersion or sprinkle curing to avoid water scarcity. It was observed that water solvent polymers can be utilized as a self-curing agent, i.e. polyethyle...
متن کامل